Spectrophotometric Assay Of Polyphenol Oxidase From Banana Lab Report

Int j food sci technol 200944840846. Phate ph 70 for use in the assay procedures.

Pdf Inhibition Of Polyphenol Oxidase In Banana Apple And

spectrophotometric assay of polyphenol oxidase from banana lab report

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Detection and identification of the polyphenoloxidase substrate of the banana.

Spectrophotometric assay of polyphenol oxidase from banana lab report. The best extraction result for the polyphenol oxidase ppo from the musa cavendishii pulp was obtained using a phosphate buffer 002 m ph 7 containing pvp 2 triton x 100 1 and ascorbic acid 001. 5 reported similar results in their studies on monoamine oxidase. Palmer banana polyphenoloxidase results extraction of soluble ppo.

Spectrophotometric assay of cytochrome c oxidase. De tergent at 1 approached the minimum concentra tion required for efficient extraction of banana. Also polyphenol oxidase i chloroplastic an enzyme involved in fruit browning is a tetramer that contains four atoms of copper per molecule and binding sites for two aromatic compounds and oxygen.

22 dogan s arslan o ozen f. Apples malus sylvestris var. This procedure not only solubilized banana ppo but also increased the activity by a factor of 15 to 25 table i.

Polyphenol oxidase ec 11031 ppo in the pulp of banana musa sapientum l was purified to 636 fold with a recovery of 30 using dopamine as substrate. Domestica are an important source of polyphenols phenolic compounds in the human diet hertog et al 1992 and a classic example of fruit susceptibility to enzymatic browning which is a major problem for the fruit processing industry coseteng and lee 1987phenolic compounds are partially responsible for color astringency bitterness flavor and. Gros michel were the source of the enzyme.

Keller pj cohen e neurath h. Chromatographic isolation further characterization and activation. Aaa group gros michel polyphenol oxidase.

Initial experiments were carried out with buffer ed homogenates 1 2 g of tissue in 20 ml of 01 m potassium phosphate ph 6 or 7 of banana pulp or peel tissue. Two active ppo isozymes were obtained applying a procedure involving dialysis stepwise precipitation deae and sephadex column chromatography. Therefore this assay improves the sensitivity of polyphenol oxidase detection over that of the direct spectrophotometric assay of quinone formation.

The purified enzyme exhibited a clear single band on polyacrylamide gel electrophoresis page. The difference in molar extinction coefficients between adduct and phenol at 300 nm ranged between 2000 and 2800 m 1cm 1 ie two times higher than those of the corresponding o quinones. All operations were conduct ed at 0 to 50 unless otherwise specified.

Ripe banana fruits musa acuminata var. Polyphenol oxidase activity of oregano in different stages. The enzyme works by catalyzing the o hydroxylation of monophenol molecules in which the benzene ring contains a single hydroxyl substituent to o diphenols phenol.

Pdf Enzyme Kinetics Teaching Using Polyphenoloxidase With A