Biotech class secondary education major in biological science doing banana tissue culture at romblon state university. Tissue culturing is the popular process of creating clones from a source plant.
Banana Tissue Culture At Home How To Do Banana Plant Tissue
how to make tissue culture banana at home
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Tissue culture of plants requires a sterile working environment to avoid contamination of the growing medium.
How to make tissue culture banana at home. There are other techniques to propagate banana plants however such as nicking corms or dividing corms. Banana tissue culture in the lab. The tissue culture banana plantlets are available in net pots with a height of 12 cm approximately with 3 4 leaves from commercial laboratories primary nurseriesthese tissue cultured plantlets are packed in open cartons and transported to the required area through trucks or minivans.
A sterile still air cabinet used to transfer plants. After your field is prepared you dig the holes 15 2ft x 15 2ft x 15 2ft. Preferably you use a pup for tissue culture rather than destroying the mother plant.
The banana crop can be grown without any pest or disease incidents with good crop management practices. It makes no difference if you want to replicate many plants from a single favorite or if you prefer to collect specimens at a local park to bring home and regrow for some garden variety. There are exceptions banana being one.
For the avid gardener knowing how to propagate plants at home through tissue culture can save a lot of money. Plant tissue culture is an abbreviation for all in vitro techniques for culturing propagating and manipulating plant celltissue or organ cultures in an aseptic environment on proper growth medium. Tissue culturing other propagation techniques of banana plants this forum is for discussing propagation techniques of banana plants.
Items needed for home tissue culture. To get good material for tc with a banana you generally dig up the corm and cut off the growing point. This is cleaned and trimmed down several times.
As we already mentioned tissue culture banana plants are disease free at the time planting. Any perspex or glass chamber with dimensions of 50 cm length 40 cm height and 40 cm depth could easily be made into a transfer cabinet. Tissue culture banana seedlings are bought when they are ready to be planted in the garden so you need to have your garden ready by the time you go to pick them from us.
Procedure of planting tissue culture banana seedlings. A fish tank on its side makes an ideal transfer cabinet. Home gardeners can purchase tissue cultured banana at plant sales if available.
Commercial tissue culture laboratories are generally equipped with laminar flow hoods and autoclaves and they operate using sterile techniques. Primary nursery tissue culture plants of banana plantlets. To avoid any pest or disease incidents after planting you can apply 400 to 450 grams of neem cake along with pit mixture before transplanting in the field.
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